Object Separation using Random-Walk Distance Map
Recipe utilizing an object separation workflow relying on the Random-Walk Distance Map. It is more robust than Separate Object for noisy segmentation or non-spherical shapes.
Object Separation workflows typically rely on a Euclidean or Chamfer Distance map for the detection of local extremities to identify the center of individual objects, and a watershed transform to identify separating lines. In Amira-Avizo-PerGeos, the module Separate Object implements such a workflow.
However, such workflows produce best results on a clean segmentation of spherical objects, but tend to yield over and/or under-splitting when the segmentation is noisy and when the shape of objects becomes elongated, flat, or non-convex.
Compared to traditional distance maps, the Random-Walk Distance Map is less sensitive to noise in the segmentation, or to the shape factor of the objects, and provides more robust results for object separation.
This Xtra encapsulates the workflow of separating a binary segmentation into individual objects in a single, simple to use image recipe. To use the recipe, make sure to invoke it from an "Image Recipe Player" module in the Project View, or from the "Image Recipe Designer" workroom.
Download a version compatible with Amira-Avizo 2022.1 HERE.
Data courtesy: Mason Dean & Ronald Seidel (MPI Postdam-Golm), David Knoetel (Zuse Institute Berlin)