Feature segmentation for non-uniform illumination and low-contrast images
The recipe lets you segment small features on images with non-uniform illumination and low contrast.
Segmentation of image captured under natural environment is often rendered ineffective due to the presence of non-uniform illumination (the average intensity fluctuates along z-axis) and low contrast. These may produce different intensity level and thus lead to inaccurate segmented small features. The recipes proposed an intensity/variance-based image correction integrated with adaptive thresholding that was able to partition small features correctly.
- Unzip Xtra_Feature-segmentation.zip file;
- Load the data and recipe in PROJECT and RECIPES view, respectively.
- Click “Run Recipe”
- Notes: The reference image in
should be a “segmentable slice” from the original image stack
Data courtesy of Yang YU